Journal:

Article Title: Pharmacological characterization of the human P2Y 11 receptor

doi: 10.1038/sj.bjp.0702909

Figure Lengend Snippet: Concentration-action curves of ATP, ADPβS and AMPαS on the IP3 and cyclic AMP accumulation in 1321N1 or CHO-K1 cells expressing the human P2Y11. The cells were incubated with ATP, ADPβS and AMPαS at various concentrations for 30 s (A) or 15 min (B). The data represent the mean±s.d. of triplicate experimental points and are representative of three independent experiments.

Article Snippet: ATP, GTP, CTP, TTP, ITP, ATPγS (adenosine 5-O-(3-thiotriphosphate)), ADPβS (adenosine 5′-O-(2-thiodiphosphate)), AMPαS (adenosine 5′-O-thiomonophosphate), BzATP (2′- and 3′-O-(4-benzoyl-benzoyl)adenosine 5′-triphosphate), A2P5P (adenosine 2′,5′-diphosphate), A3P5P (adenosine 3′,5′-diphosphate), A3P5PS (adenosine 3′-phosphate 5′-phosphosulphate), the diadenosine polyphosphates (AP 4 A, AP 5 A and AP 6 A) and pertussis toxin were obtained from Sigma Chemical Co. (St. Louis, MO, U.S.A.).

Techniques: Concentration Assay, Expressing, Incubation

Journal:

Article Title: Pharmacological characterization of the human P2Y 11 receptor

doi: 10.1038/sj.bjp.0702909

Figure Lengend Snippet: Concentration-inhibition curves of ADPβS and AMPαS on the IP3 or the cyclic AMP accumulation by ATP respectively in 1321N1 or CHO-K1 cells expressing the human P2Y11 receptor. Transfected cells were incubated in the presence or the absence (CONT) of ATP (100 μM) and various concentrations of ADPβS or AMPαS for 30 s (A) or 15 min (B). The data are the mean±s.d. of triplicate experimental points obtained in an experiment representative of three.

Article Snippet: ATP, GTP, CTP, TTP, ITP, ATPγS (adenosine 5-O-(3-thiotriphosphate)), ADPβS (adenosine 5′-O-(2-thiodiphosphate)), AMPαS (adenosine 5′-O-thiomonophosphate), BzATP (2′- and 3′-O-(4-benzoyl-benzoyl)adenosine 5′-triphosphate), A2P5P (adenosine 2′,5′-diphosphate), A3P5P (adenosine 3′,5′-diphosphate), A3P5PS (adenosine 3′-phosphate 5′-phosphosulphate), the diadenosine polyphosphates (AP 4 A, AP 5 A and AP 6 A) and pertussis toxin were obtained from Sigma Chemical Co. (St. Louis, MO, U.S.A.).

Techniques: Concentration Assay, Inhibition, Expressing, Transfection, Incubation